• Quantitative Vitamin D test version configured for cube readers
  • Quantitative read out using a calibrated cube reader
  • Results in 10 minutes
  • CE Mark
  • Range: 10 – 250 nano mol/L
  • Accuracy: 94% correlation with gold standard LC/MS blood tests


  • Quantitative Vitamin D test version configured for testing laboratories
  • Two dimensional image analysis of test cassette
  • Results in 10 minutes
  • CE Mark
  • Range: 10 – 250 nano mol/L
  • Accuracy: 91% correlation with gold standard LC/MS blood tests


  • Semi-quantitative test version providing a color-coded indication of Vitamin D levels
  • Results in 10 minutes
  • CE Mark

Quick and easy Point of Care Testing

Next Generation -Test4D Technology - Absolutely Better Choice

PARAMETERS Test4D Next Generation Technology State of the Art and First to Market Technology OTHER PLAYERS IN THE MARKET


One monoclonal antibody is industry best practice for small molecules Uses two antibodies - obsolete method for small molecules
Proprietary Patented Antibody, US 2014, IND 8681 Non-verified antibody sources

Sample Size

Fixed volume 3-4 drops - high variance

Sample Prep

No Sample prep Sample preparation required

Test Formats - Multiple Choices

Quantitative Test Reports - Reader Version targeted for Hospitals and Large labs Not Available
Quantitative - Cube Reader Available Quantitative - Cube Reader only
Qualitative/Semi-Quantitative Available Not Available

About 1 billion people worldwide are Vitamin D deficient.

Our testing kits can determine Vitamin D levels in as little as 10 minutes and is very simple to administer.

These second-generation Vitamin D tests are complemented by NanoSpeed’s widely sold first generation test, Test4DTM, which qualitatively indicates Vitamin D deficiency/sufficiency levels rapidly in a blood sample.

NanoSpeed’s clients also buy our Test4CaTM, calcium test, as an extremely valuable companion test to our Vitamin D tests, since excessive 25(OH) Vitamin D levels can cause excessive levels of calcium in the blood (hypercalcemia), which has symptoms including feeling tired, thirsty or sick; pain in muscles or bones; or passing urine often.

News & Events

NanoSpeed Diagnostics: 2016 Alberta Export Award Finalist

EDMONTON, Alberta – (November 2, 2016) – NanoSpeed Diagnostics Inc. (“NanoSpeed” or “the Company”) is pleased to announce that it was chosen to be a finalist in the category of Advanced Technology and Innovation by the Alberta Export Awards presented by Edmonton Economic Development Corporation and the Edmonton International Airport. The Company’s Test4D™ product is […]

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NanoSpeed Diagnostics and Test4D™ achieves major sales milestone

EDMONTON, Alberta – NanoSpeed Diagnostics Inc. (“NanoSpeed” or  “the Company”) is pleased to announce that Test4D™, the world’s first point-of-care test for vitamin D sufficiency, has reached sales of one million (1,000,000) tests since the product launch in 2013. “The achievement of this milestone is both a demonstration of the strong demand for on-the-spot point […]

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NanoSpeed Diagnostics enters into Research Collaboration with Dynalife Dx

EDMONTON, Alberta – (January 18, 2016) – NanoSpeed Diagnostics Inc. (“NanoSpeed”) is pleased to announce a new multi-year research collaboration with Dynalife Dx Inc. (“Dynalife”) to develop new point of care tests to help patients obtain faster information on their own health. “We strongly believe that point of care diagnostics are the simplest and fastest way […]

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NanoSpeed Announces Global Distribution Partnership with DiaSys Diagnostics Systems for Test4D™

Edmonton, Alberta – (March 2, 2015) – NanoSpeed Diagnostics, Inc. (“NanoSpeed”) is pleased to announce an expansion of their exclusive distribution agreement with global diagnostics and instrumentation distributor, DiaSys Diagnostics Systems GmbH (“DiaSys”), to market their 25-OH Vitamin D deficiency test kit. Under the broadened agreement, DiaSys will market the point-of-care vitamin D test under their […]

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